Anti-CD257 (BAFF) [Tabalumab] Ab00958-10.0 from Absolute Antibody

Product Specs
ItemAnti-CD257 (BAFF) [Tabalumab]
CompanyAbsolute Antibody
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAb00958-10.0
Quantity200 µg
ApplicationsWB, FC, IP, ELISA, Neutralisation, IF, Functional Studies
Conjugate/TagUnconjugated, AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
TargetCD257 (BAFF)
IsotypeIgG1
CloneTabalumab
HostHuman
ImmunogenMonoclonal antibodies were generated by immunizing human immunoglobulin transgenic mice with soluble human BAFF (amino acids 133–285). Eight mice producing serum antibody titers to human BAFF were injected intravenously with 10 µg human BAFF in phosphate buffered saline. The spleen was harvested 3 days later from each mouse and fused with myeloma cells. 20 Hybridomas were tested for specific binding to human BAFF and to make sure they were expressing human immunoglobulin heavy and light chains by enzyme-linked immunosorbent assay (ELISA). The hybridoma producing the antibody that eventually became tabalumab was a human IgG1 isotype. The antibody was then engineered to a human IgG4 isotype for complete neutralization of BAFF without depletion of BAFF-expressing cells
NCBI Full Gene NameTNF superfamily member 13b
NCBI Gene AliasesBAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF20, TNLG7A, ZTNF4
ClonalityMonoclonal
ReactivityHu, Rb, CyMk
Research AreaAutoimmune; biosimilar
PurityProtein A affinity purified